Full-Genome Sequence of Human Betacoronavirus 2c Jordan-N3/2012 after Serial Passage in Mammalian Cells
Identifieur interne : 001C49 ( Main/Exploration ); précédent : 001C48; suivant : 001C50Full-Genome Sequence of Human Betacoronavirus 2c Jordan-N3/2012 after Serial Passage in Mammalian Cells
Auteurs : Kenneth G. Frey ; Cassie L. Redden ; Kimberly A. Bishop-Lilly ; Reed Johnson [États-Unis] ; Lisa E. Hensley [États-Unis] ; Kanakatte Raviprakash [États-Unis] ; Thomas Luke ; Tad Kochel [États-Unis] ; Vishwesh P. Mokashi [États-Unis] ; Gabriel N. Defang [Égypte]Source :
- Genome Announcements [ 2169-8287 ] ; 2014.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is the etiologic agent of a highly lethal pneumonia. Here, we report the full-genome sequence of the Jordan-N3/2012 strain after serial passage in two distinct mammalian cell lines. The genome exhibits noteworthy stability, which may inform the development of vaccines and therapeutics used to treat infection with this virus.
Url:
DOI: 10.1128/genomeA.00324-14
PubMed: 24874668
PubMed Central: 4038873
Affiliations:
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Mokashi</name><affiliation wicri:level="2"><nlm:aff id="aff1">Naval Medical Research Center, NMRC-Frederick, Fort Detrick, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Naval Medical Research Center, NMRC-Frederick, Fort Detrick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Defang, Gabriel N" sort="Defang, Gabriel N" uniqKey="Defang G" first="Gabriel N." last="Defang">Gabriel N. Defang</name><affiliation wicri:level="1"><nlm:aff id="aff5">Viral and Zoonotic Diseases Research Program, U.S. Naval Medical Research Unit 3, Abbassia, Cairo, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Viral and Zoonotic Diseases Research Program, U.S. Naval Medical Research Unit 3, Abbassia, Cairo</wicri:regionArea><wicri:noRegion>Cairo</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genome Announcements</title><idno type="eISSN">2169-8287</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is the etiologic agent of a highly lethal pneumonia. Here, we report the full-genome sequence of the Jordan-N3/2012 strain after serial passage in two distinct mammalian cell lines. The genome exhibits noteworthy stability, which may inform the development of vaccines and therapeutics used to treat infection with this virus.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="De Graaf, M" uniqKey="De Graaf M">M de Graaf</name></author><author><name sortKey="Lauber, C" uniqKey="Lauber C">C Lauber</name></author><author><name sortKey="Bestebroer, Tm" 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